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Biology Biotechnology Principles and Processes NCERT Single Correct MCQ
Published on: August 13, 2026

pBR322 which is frequently used as a vector for cloning gene in E. coli is a/an

A
original bacterial plasmid
B
modified bacterial plasmid
C
viral genome
D
transposon

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Text Solution

Verified by Experts
The correct answer is:
B

pBR322 is an artificially modified plasmid derived from naturally occurring plasmids of E. coli. It was developed by Bolivar and Rodriguez and is widely used as a cloning vector.

It contains:

• ori (origin of replication)

• ampR (ampicillin resistance gene)

• tetR (tetracycline resistance gene)

• Several unique restriction sites

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